This is a work-in-progress documentation for laser-cut and 3d-printed fluorescence plate reader with box and raspberry pi camera. The assembly instructions will be added soon. Here is our version repository: https://github.com/SynBioUC/Raspiscope. This work is conducted in the synthetic biology network, Santiago, Chile.
This is the second "stable" release of the digital 3d-printed open flexure microscope; ongoing development lives on the Github page https://github.com/rwb27/openflexure_microscope and the accompanying paper is available at http://dx.doi.org/10.1063/1.4941068 (the paper is open-access). This microscope is available through http://www.waterscope.org/ as a kit.
A handy dispenser for sterile ball bearings used in some library preparation/DNA extraction protocols, releasing a single ball bearing per press of the plunger. Without this device, this stage in the protocol requires a scientist to retrieve tiny ball bearings from a bag, one by one, while wearing gloves and maintaining sterile technique - a substantial drain on time and patience. It is easily capable of holding more than 100 ball bearings. A handy dispenser for sterile ball bearings used in some library preparation/DNA extraction protocols, releasing a single ball bearing per press of the plunger. Without this device, this stage in the protocol requires a scientist to retrieve tiny ball bearings from a bag, one by one, while wearing gloves and maintaining sterile technique - a substantial drain on time and patience. It is easily capable of holding more than 100 ball bearings. This project was designed in DesignSpark Mechanical.
Optomechanics is a crucial part of any microscope; when working at high magnification, it is absolutely crucial to keep the sample steady and to be able to bring it into focus precisely. Accurate motion control is extremely difficult using printed mechanical parts, as good linear motion typically equires tight tolerances and a smooth surface finish. This design for a 3D printed microscope stage uses plastic flexures, meaning its motion is free from friction and vibration. It achieves steps well below 100nm when driven with miniature stepper motors, and is stable to within a few microns over several days. This design aims to minimise both the amount of post-print assembly required, and the number of non-printed parts required - partly to make it as easy as possible to print, and partly to maximise stability; most of the microscope (including all the parts with flexures) prints as a single piece. The majority of the expense is in the Raspberry Pi and its camera module; the design requires only around 100g of plastic and a few nuts, bolts and other parts. The optics module (containing the camera and lens) can be easily swapped out or modified, for example to add epifluorescence or change the magnification. The location with OpenSCAD design files can be found here: https://github.com/rwb27/openflexure_microscope Associated Publication: http://dx.doi.org/10.1063/1.4941068 . Other projects that inspired this work: OpenLabTools microscope http://www.openlabtools.org/.
KORUZA is a low cost, open source and open hardware, wireless optical system, making the free space optical (FSO) technology available to masses and providing an alternative to Wi-Fi networks. 1Gbps/10Gbps networking connectivity for locations up to 150 m apart, using an eye-safe infrared light beam. http://koruza.net/
This is a documentation to build a DIY enclosure for the 3D printer model Rostock Max V2 (should also work for V3) that is part of the RepRap family of 3d printers and sold by SeeMeCNC and through various resellers. Enclosures are typically self-built, because a USA patent (attached) seems to be an obstacle for most providers to sell them as standard accessory. This project uses PLA and ABS prints with the printer itself, laser cut acrylic sheets and a few metric of-the-shelf components only. Enclosures are desirable to improve the quality of especially ABS prints by increasing the temperature of the print environment and by preventing quick temperature changes e.g. by drafts. ABS print especially benefit from both a lower temperature difference between the heated printer bed and the environment because the material shrinks more than PLA when cooling down, which leads to warping and splitting of prints. Please share if you implement further improvements for this enclosure. My design files can be seen and modified online in the Onshape project: https://cad.onshape.com/documents/d590c2db52ca428e97d68baa/w/b00a0e533d044792ac7fa99f/e/468f1fb1db04
The fruit fly, Drosophila melanogaster, is one of the most important model organisms in biological research. Maintaining stocks of fruit flies in the laboratory is labour-intensive. One task which lends itself to automation is the production of the vials of food in which the flies are reared. Fly facilities typically have to generate several thousand vials of fly food each week to sustain their fly stocks. The system presented here combines a cartesian coordinate robot with a peristaltic pump. The design of the robot is based on the Routy CNC Router created by Mark Carew (http://openbuilds.org/builds/routy-cnc-router-v-slot-belt-pinion.101/), and uses belt and pully actuators for the X and Y axes, and a leadscrew actuator for the Z axis. CNC motion and operation of the peristaltic pump are controlled by grbl (https://github.com/grbl/grbl), an open source, embedded, high performance g-code parser. Grbl is written in optimized C and runs directly on an Arduino. A Raspberry Pi is used to generate and stream G-code instructions to Grbl. A touch screen on the Raspberry Pi provides a graphical user interface to the system. This documentation describes the design and build of the hardware. Software source code and operating instructions can be found here: https://github.com/WaylandM/fly-food-robot
Gel electrophoresis systems are one of the most common tools of scientists working with DNA in experiments. Yet existing commercial solutions are expensive and non-customizable. Gel electrophoresis systems do not follow many standards so with the open source parametric system presented here, there is nothing that stops you from building a gel system in the size that most suits your needs - for a fraction of the prize of a commercial system. This gel system was designed to be of research grade quality, safe, easy to build and easy to modify. This documentation contains construction files for building a small gel electrophoresis system with a gel size of 100x70mm and two comb slots. This documentation also explains how to customise the online design around 10 main and 7 minor parameters (without installing any software) and how to easily download and make the modified gel box.
These bricks were designed as part of a 3d printer enclosure and can easily be reused for any other hardware.
Microfluidics flow control system